Laboratorio della sezione di Genetica Agraria


Attrezzature principali - Main facility

Typhoon 9200 Versatile scanner capable of detecting fluorescent, chemiluminescent as well as radioactive labelled samples by handling gel sandwiches, agarose and polyacrylamide gels, membranes, microplates, and macroarrays.

Multiprobe II Expanded Multifunctional liquid handling robot with eight independent pipetting tips for the automation of liquid handling in microtiter plates, tubes, etc. It can be used for vacuum and magnetic based isolation procedures, popular techniques are RNA and DNA extractions, PCR and sequence reactions setup and regridding of 96 to 384 well samples.

QPix2XT colony picker. The Qpix2XT is a high-speed, high-precision robot, ideal for high-throughput applications in 96-well and 384-well microtiter plate formats, with data tracking. The main application is colony picking, it picks approximately 3,500 colonies per hour to inoculate standard size or deep well plates containing liquid growth media. The machine is capable to perform: replication (it allows for library copying, compression (96 to 384 wells) and expansion (384 to 96 weels), reArraying (rearranging clones, or “hit-picking”, from library according to user input files) and gridding (to make high-density macro-arrays on 22 x 22 cm nylon membranes or agar plates).

Omnilog PM Phenotype Microarray Scanner. An integrated system of cellular assays, instrumentation, and bioinformatics software, monitoring thousands of phenotypes simultaneously.

iCycler iQ Real-Time PCR Detection System Thermal cyclers: MJTetrad Thermal cycler used for high-throughput PCR and cycle sequencing applications. The instrument has a capacity of 3 96-well and 1 384-well PCR plates; 5 thermal cyclers all capable of handling microtiter plates.

AKTA FPLC©XY System Fast Performance Liquid Chromatography. System for the rapid purification of proteins at low to medium pressures greatly increases protein purification efficiencies Cary Eclipse Fluorescence spectrophotometer . Computer controlled fluorescence spectrophotometer with measurement modes for fluorescence, phosphorescence, chemiluminescence and bioluminescence

QELS-DAWN System measures molar mass, the chemical union of two identical molecules using multi-angle light scattering

JASCO J-810 Circular Dichroism spectrometer. Equipped with a computer-controlled Peltier device and a two-syringe titrator is used to obtain information about protein secondary structure. Centrifuges, Speed Vacs, and Shakers all capable of handling microtiter plates.


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